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DNA must be administered, reach the damaged cells, enter the cell and either express or disrupt a protein. The initial approach incorporated DNA into an engineered virus to deliver the DNA into a chromosome.Naked DNA approaches have also been explored, especially in the context of vaccine development.The target DNA is then washed and the labeled probes that didn't hybridize are removed.The target DNA is then analyzed for the presence of the probe via radioactivity or fluorescence.The first attempt at modifying human DNA was performed in 1980 by Martin Cline, but the first successful and approved nuclear gene transfer in humans was performed in May 1989.

Writing in Nature in 1961, William Astbury described molecular biology as: "..so much a technique as an approach, an approach from the viewpoint of the so-called basic sciences with the leading idea of searching below the large-scale manifestations of classical biology for the corresponding molecular plan.

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